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Omnes, Marie-helene; Temey, Yann; Daniel, Jean-yves; Nicolas, Jean-louis. |
Bacterial infections have been suspected to be one cause of variable success in the incubation of turbot (Scophthalmus maximus) eggs. The assessment of bacterial flora (bacterial environment) associated with ova, sperm and developing eggs was conducted just at stripping gametes and during incubation. Gametes were collected in sterile conditions before fertilization. Eggs were held in an open-circulation 401 incubator, at 13 degree C during 6 days until hatching. Samples of those were collected every day. They were rinsed several times with sterile seawater, counted and grown. Viable counts of bacteria were determined on two media, Zobell and TCBS agar. Epibiotic bacteria on the surface of the eggs and impact on the chorion were observed at scanning... |
Tipo: Text |
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Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/00199/31015/29425.pdf |
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